For the extrusion process of TC32 titanium alloy, the evolution laws of microstructure and mechanical properties of TC32 titanium alloy extruded bars under the extrusion ratios of 4, 6 and 8 and the extrusion temperatures of (Tβ-60)-(Tβ+10) ℃ were investigated. The results show that extrusion ratio and extrusion temperature significantly aftect the microstructure and mechanical properties of alloy. When extruded in the α+β two-phase temperature region, a duplex structure is formed. As the extrusion ratio increases, the amount of equiaxed α phase decreases, and the content of short-rod-shaped secondary α phase increases and continues to coarsen, which increases the tensile strength and yield strength by 14 MPa and 4 MPa, respectively, while the elongation remains at 18%. When extruded in the β single-phase temperature region, the alloy exhibits a basket-weave microstructure, in which the length of lamellar α phase increases with the increasing of extrusion ratios, resulting in a decrease of 17 MPa and 20 MPa in tensile strength and yield strength, respectively, while the elongation increases by 2%. When the extrusion ratio remains constant, the tensile strength of alloy gradually increases with the increasing of extrusion temperature, while the yield strength and elongation first increase and then decrease. The research results provide a technical reference for the comprehensive application of TC32 titanium alloy.